Lourdes Georgina IGLESIAS-ANDREU
Instituto de Biotecnología y Ecología Aplicada (INBIOTECA), Universidad Veracruzana, Avenida de las Culturas Veracruzanas 101, Colonia Emiliano Zapata, C.P. 91090, Xalapa, Veracruz – Mexico

Enrique FAVIÁN-VEGA
Instituto de Biotecnología y Ecología Aplicada (INBIOTECA), Universidad Veracruzana, Avenida de las Culturas Veracruzanas 101, Colonia Emiliano Zapata, C.P. 91090, Xalapa, Veracruz – Mexico

https://doi.org/10.47743/jpd.2021.28.1.897

Keywords: conservation, cycad, dioecious, molecular markers, sex.

Abstract: Zamia inermis is a dioecious cycad endemic to the state of Veracruz that is on the risk extinction. Sex-specific markers are important in understanding the mechanism of sex determination. However, since little is known about the mechanism of sex determination in Z. inermis, we proposed looking for Inter-simple Sequence Repeats (ISSR) markers that could be linked to sexual expression in this species. Using DNA bulk samples of male and female genotypes and 6 ISSR primers, a female marker (~867 bp) was identified with primer ISSR-18, which was present in 66% of the DNA mixtures of the female genotypes analyzed. The results of the Principal Coordinate Analysis performed revealed a tendency for clustering of genotypes of the same sex.

How to cite this article:
IGLESIAS-ANDREU L. G. & FAVIAN-VEGA E. 2021. Identification of sex in Zamia inermis using ISSR markers. J. Plant Develop. 28: 33-40.
https://doi.org/10.47743/jpd.2021.28.1.897

[PDF]