Ion ROSCA 
“Alexandru Ciubotaru” National Botanical Garden (Institute)
– Chisinau, Republic of Moldova
Aliona GLIJIN
“Alexandru Ciubotaru” National Botanical Garden (Institute)
– Chisinau, Republic of Moldova
Nina CIORCHINA
“Alexandru Ciubotaru” National Botanical Garden (Institute)
– Chisinau, Republic of Moldova
Maria TABARA
“Alexandru Ciubotaru” National Botanical Garden (Institute)
– Chisinau, Republic of Moldova
Alina CUTCOVSCHI-MUSTUC
“Alexandru Ciubotaru” National Botanical Garden (Institute)
– Chisinau, Republic of Moldova
Tudor RALEA
Institute of Genetics, Physiology and Plant Protection –
Chisinau, Republic of Moldova
Nina ZDIORUC
Institute of Genetics, Physiology and Plant Protection –
Chisinau, Republic of Moldova
Alexandru MÎRZA
“Alexandru Ciubotaru” National Botanical Garden (Institute)
– Chisinau, Republic of Moldova
https://10.47743/jpd.2022.29.1.908
Keywords: 'Arapaho', ascorbic acid, biochemical
parameters, blackberry, 'Melana', tannins, 'Thornfree', titratable
acidity, 'Triple Crown', reducing sugars.
Abstract: The goal of the research was the quantitative
determination of biochemical compounds (ascorbic acid, reducing
sugars, tannins and titratable acidity) in Rubus fruticosus
L. fruits – blackberries ('Melana', 'Triple Crown', 'Arapaho'
and 'Thornfree'), propagated by tissue culture and cultivated
under the ecological conditions of the Republic of Moldova. The
spectrophotometric determination of ascorbic acid revealed that
the highest content of this phytocompound was quantified in the
'Arapaho' blackberries, 48.28 mg/100 g, followed by the 'Thornfree'
cultivar (41.69 mg/100 g), which is an amount about twice as high
as in the 'Melana' blackberries (23.8 mg/100 g). The high amount
of vitamin C in the researched blackberries makes them comparable
to raspberries and gooseberries. The content of reducing sugars
varied between 4.72% and 7.26%. The 'Triple Crown' cultivar was
characterized by the maximum amounts of these biochemical compounds,
and the lowest amounts were found in the fruits of the 'Thornfree'
blackberry cultivar. The evaluation of the titratable acidity
showed that the highest index of this parameter was recorded in
the 'Melana' blackberries (0.74% / 0.77% as compared with malic
/ citric acid. The quantification of the tannins revealed that
the fruits of the 'Triple Crown' cultivar have the lowest tannin
content (1.97%), the amount being more than twice lower than the
maximum amount (4.16%), recorded in the blackberries of the 'Thornfree'
cultivar. The results of the study led to the conclusion that
the fruits of Rubus fruticosus L., obtained from plants
which had been micropropagated in the Embryology and Biotechnology
Laboratory (NBGI) and grown under the conditions of the Republic
of Moldova are a good source of biologically active substances
and can be proposed for commercial production as a promising organic
food product.
How to cite this article:
ROSCA I., GLIJIN A., CIORCHINA N., TABARA M., CUTCOVSCHI-MUSTUC
A., RALEA T., ZDIORUC N. & MÎRZA A. 2022. The quantification
of some bioactive compounds in the fruits of four blackberry (Rubus
fruticosus L.) cultivars, propagated by tissue culture. J.
Plant Develop. 29: 55-68
https://doi.org/10.47743/jpd.2022.29.1.908
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